Allergy
The Allergy unit collaborates with pediatricians and allergists, among other specialists, in:
- The diagnosis of allergic pathology by quantifying parameters such as total IgE, Eosinophil Cationic Protein, Tryptase, and Histamine through various types of samples and allergy tests.
- The identification of possible allergens responsible for the allergic pathology by studying the presence or absence of Specific IgE against a wide range of respiratory allergens, food allergens, some occupational allergens, drug allergens, insect venoms, and recombinant allergens.
- The monitoring of patients undergoing Hyposensitization Therapy.
Additionally, it collaborates with gastroenterologists and nutritionists by studying food intolerances to improve the quality of life of patients with gastrointestinal disorders and other associated pathologies.
Fluoroimmunoassay
- Detection of specific IgE against native and recombinant allergens
- Detection of specific IgG against native and recombinant allergens
- Detection of specific IgG4 against native and recombinant allergens
- Quantification of tryptase and eosinophil cationic protein
Enzyme immunoassay
- Histamine quantification in plasma and urine
Inmunoblot
- Food intolerance
Micro-array
- Detection of specific IgE against recombinant allergens
Functional essays
- Cell activation by allergens
- Histamine release test
Hormones
The Hormones unit offers specialist doctors a reliable tool that allows them to approach, from an analytical perspective, the different functional aspects of pathologies related to their respective clinical areas, mainly endocrinology, oncology, and prenatal diagnosis.
In the field of endocrine pathologies, the unit routinely performs a wide range of serum, plasma, urine, and saliva parameters that allow assessment of the proper functioning of all endocrine glands and, at the same time, through functional tests, the correct status of all hormonal axes.
Oncology mainly focuses on the determination of tumor markers that enable the clinical oncologist, along with other parameters, to diagnose, characterize, treat, and monitor the patient's progress.
Related to prenatal diagnosis, through biochemical markers and together with ultrasound markers, it aims to individually establish the risk of pregnancies affected by chromosomal abnormalities in the first trimester and chromosomal abnormalities and neural tube defects in the second trimester.
The different clinical analysis techniques we use are the following:
Radioimmunoassay (gamma/beta)
- Hormone analysis
- Detection of antibodies against hormone receptors Vitamins and cofactors
Sistemas automatizados quimioluminiscentes
- Preeclampsia screening
- Hormone study related to fertility
- Tumor markers
- Detection of antibodies against hormone receptors
Enzyme immunoassay
- Hormone analysis
Inmunology
The Echevarne Laboratory Immunology Unit provides support to professionals from various medical specialties in the diagnosis, monitoring, and progression of different pathologies, including those of autoimmune, infectious, hemato-oncological, rheumatic, and immunodeficiency origin, among others.
Within the scope of infectious serology, this Unit of the Clinical Analysis Area has numerous decision algorithms to facilitate the distinction between current/recent infections and the presence of residual antibodies (immunity), especially in the case of serologies related to pregnancy. Immunology has different analysis methods available for the same test, aiming to offer a better understanding of the result.
In autoimmunity, there is a wide range of tests covering from non-organ-specific antibodies to multiple specific antigens. The presence of antibodies against tissue and organ-specific antigens is detected by indirect immunofluorescence on Hep2 cells, Crithidia Luciliae, or tissue preparations, reserving ELISA, RIA, and Immunoblot techniques for the detection of antibodies against specific antigens.
Regarding immunodeficiencies and hemato-oncological diseases, techniques converge for the phenotyping of hematological cells, quantification of lymphoid cells, immunoglobulins, complement factors, and plasma proteins.
Detección de autoanticuerpos mediante fluorescencia Indirecta (IFI)
Specific antibody detection to neuronal antigens (immunoblot and IFA)
Detección de anticuerpos relacionados con enfermedades autoinmunes sistémicas (IFI, ELISA, multiplex e inmunoblot)
Detección de anticuerpos relacionados con enfermedades autoinmunes organoespecíficas (IFI, ELISA e inmunoblot)
Serology infectious
- Viral, bacterial, and parasitic serology by chemiluminescence, multiplex, and IFA
Flow cytometry
- Lymphocyte populations Leukocyte antigen study (PNH, leukemia typing) HLA B27 antigen study Fertility studies Diagnosis of autoimmune hemolytic anemia Follow-up of borrelia infection ILMI profile
Inmunoquímica
- Quantification of Immunoglobulins and plasma proteins
Enzyme immunoassay and multiplex
- Proteins related to inflammation Osteoporosis